The ICSI procedure entails the deposition of a single sperm into the main body of the egg (cytoplasm) thus bypassing the outer layers of the egg (zona pellucida). The whole procedure is performed in the lab by the embryologists using special equipment, for egg and sperm manipulation, under the control of high-powered microscopes.

ICSI is usually carried out in cases of severe male infertility (low number of active sperm or increased number of abnormal shape) and is also suggested in couples who have undergone multiple conventional IVF cycles with low fertilization rate. Finally, retrieval of low number of oocytes, the existence of sperm with no acrosome unable to penetrate the oocyte or oocytes with thick zona pellucida represent further indications for the performance of this procedure.


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IVF is one of the most frequently used techniques in assisted reproduction. It consists of bringing the egg and the sperm together to ensure that both genomes combine. When the semen sample is normal and there is a history of earlier fertilization, “conventional” insemination is performed. This consists in placing the eggs in contact with a specific concentration of processed sperm (one hundred thousand sperm with good motility) in culture medium and incubating them at 37ºC until fertilization is observed.

When the sperm sample is of low quality or there is no prior history of fertilization, the insemination technique used is IntraCytoplasmic Sperm Injection (ICSI).
ICSI is a highly sophisticated technique, requiring a great degree of precision, expertise and experience by the embryologist. It is the introduction by injection of a “live” sperm into the cytoplasm of the egg.
In the case of the Egg Donor Program, ICSI is absolutely necessary, regardless of the quality of the semen, in order to ensure a high rate of fertilization.


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Today there are thousands of women who, for many different reasons need donated eggs in order to conceive. The egg donation is an anonymous procedure in which the donor provides oocytes to the receiving couple.

Donated eggs from volunteers are fertilized with sperm from the recipients partner. After fertilization, the resulting embryos are transferred to the receiving patient following gestation.

We run a range of donor egg programs designed to meet the needs of women in both groups, following the recommendations of the effective legislation. In any case we are working to provide recipients with a matched donor through the highest standars of medical care and treatments. Notably, unlike other programs, we do not advertise, recruit or solicit Egg Donors. All of the Donors in our program have located us as a result of their own diligence and research. You therefore can be assured that these woman are mature, thoughtful and have considered all the ramifications involved.


Partners > Embio Medical center

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